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Complete Program DehaloCon II

Sunday, March 26 (Location - Helmholtz Center for Environmental Research - UFZ Leipzig, Kubus, Permoserstr. 15)


3pm: OPTIONAL: Guided Tour through the Helmholtz Center for Environmental Research

DehaloCon II Registration (UFZ Leipzig Kubus Foyer)

5pm: Introduction DehaloCon II

Lorenz Adrian, Gabriele Diekert, Tobias Goris, Lukas Wick

Holger Weiß (UFZ Leipzig) - 20 years of research and development in contaminated megasite
management - lessons learned after Germany´s reunification 

Welcome Reception, Kubus Foyer, UFZ Leipzig


Monday, March 27 (University Leipzig, Seminar Building Augustusplatz, Seminar Room 2)


8:30 - 9:50am: Biochemistry and Architecture of Organohalide Respiratory Chains

Lorenz Adrian (UFZ Leipzig) - Electron paths in reductive dehalogenation: electron chain architecture and B12-dependent substrate reduction

Katja Seidel (UFZ Leipzig) - Multienzyme complexes in Dehalococcoides mccartyi strain CBDB1

Gary Sawers (Uni Halle) - A Hydrogen-Oxidizing, Organohalide-Reducing Respiratory Complex in Dehalococcoides mccartyi

Yaqing Liu (UFZ Leipzig) - Characterisation of anaerobic biotransformation of hexachlorocyclohexane by Dehalococcoides mccartyi and an enrichment culture

20 min Coffee Break, Foyer in Front of Seminar room

10:10-11:20 am: Regulation of Organohalide Respiratory Chains

Ute Lechner (Uni Halle) - Control of rdhA gene transcription by RdhR regulators in Dehalococcoides mccartyi

Franziska Greiner-Haas (Uni Halle) - Role of the Two-Component System RdhS(79)P(78) in rdhA Gene     Expression in Dehalococcoides mccartyi CBDB1

Mathilde Willemin (EPFL Lausanne) - Hybrid proteins for the screening of transcription regulators of reductive dehalogenase gene clusters

Flash Poster Presentations 2 mins each

Nadya Krumova (Uni Halle) - Enrichment of a Hydrogen-Dependent, 1,2,3-TCB-Dechlorinating Activity Catalyzed by a Respiratory Protein Complex from Dehalococcoides mccartyi
Jens Esken (Uni Jena) - What triggers transcriptional regulation of organohalide respiration in Sulfurospirillum ssp.?
Sebastian Keller (Uni Jena) - Characterization of a novel L-serine O-phosphate decarboxylase involved in norcobamide biosynthesis in Sulfurospirillum multivorans
Christina Lihl (Helmholtz Zentrum München) - Comparison of TCE and cis-DCE Reductive Dechlorination with Different Bacterial Cultures via Dual Element Isotope Analysis (δ13C/δ37Cl)

11:30 to 12:30: Lunch break Mensa Augustusplatz

12:30 – 1:40pm: Poster session I (Foyer in Front of Lecture Hall 2)

1:40 – 5pm: Structure Function Relationships of B12 enzymes

Fraser Armstrong (University of Oxford) - The electrocatalytic activity of reductive dehalogenases

Torsten Schubert (Uni Jena) - A case report on the mechanism of enzymatic cobamide-dependent reductive dehalogenation

Benjamin Heckel (Helmholtz Zentrum München) - Which Mechanisms of Chlorinated Ethene Transformation Lie at the Heart of Reductive Dehalogenases?

Cindy Kunze (Uni Jena) - Site-directed mutagenesis of two reductive dehalogenases known to catalyze either halogen substitution or dihaloelimination

20 min break

Catherine Drennan (Massachusetts Institute of Technology) - Molecular Basis for cobalamin-dependent RNA modification

Jun Yan (University of Tennessee) - TceA of Dehalococcoides mccartyi is a Vinyl Chloride-to-Ethene Reductive Dehalogenase

Masafumi Yohda (Tokyo University of Agriculture and Technology) - Functional expression and characterization of reductive dehalogenases

6pm: Zoo Leipzig,  Mixer in Gondwanaland


Tuesday, March 28 (University Leipzig, Seminar Building Augustusplatz, Seminar Room 2)


9 to 12am: Non-OHR Non-respiratory Reductive Dehalogenation

Jiandong Jiang (Nanjing Agricultural University) - Reductive dehalogenation of halogenated aromatics in aerobes and evolution of the reductive dehalogenase gene clusters on plasmid pBHB

Fraser Collins (University of Manchester) - Biochemical characterization of the electron transfer proteins associated with NpRdhA

Oliver Tiedt (Uni Freiburg) - Carbon-halide cleavage by ATP-dependent benzoyl-CoA reductases or by enoyl-CoA hydratases during complete anaerobic degradation of haloaromatic compounds          

                Coffee break

Gao Chen (University of Tennessee) - Interspecies hydrogen transfer enables concomitant dichloromethane and chloromethane degradation in an anaerobic consortium

Matt Lee (University of New South Wales) - Dichloromethane fermentation by a novel member of Peptococacceae

Flash Poster Presentations 2 mins each

Alba Trueba-Santiso (Universitat Autonoma de Barcelona) - Dichloromethane fermentation by an enrichment culture containing a Dehalobacterium sp: molecular and carbon isotopic analysis.
Zeev Ronen (Ben-Gurion University of the Negev) - Determining the fate and transport of a persistant halogenated compound in contaminated groundwater
Marie Markantonis (Università degli Studi di Milano) - Assessment of natural and enhanced reductive dehalogenation of chlorinated aliphatics in a contaminated aquifer under remediation
Marco Rocca (University of Bologna) - Identification of Multiple Dehalogenating Capabilities and RDases in Marine Dehalococcoidia
Bruna Matturo (IRSA CNR, Italy) - High-throughput sequencing analysis revealed Dehalobium enrichment during PCE-to-ethene dechlorination in marine sediments
Mohammed Asad (UFZ Leipzig) - Characterization of anaerobic biotransformation of
Thomas Kruse (University of Wageningen) - The Desulfitobacterium genome
Patrick Lohmann (UFZ Leipzig) - Monitoring enzyme-substrate binding by Thermal Proteome Profiling to elucidate regulation of the reductive dehalogenases
Shujuan Lian (UFZ Leipzig) - Tracking reductive dechlorination of Hexachlorocyclohexane (HCH) in biogas reactors via compound-specific stable isotope analysis (CSIA)
Johannes Büsing (Uni Tübingen) - Stable carbon isotope fractionation of tetrachloroethene in mixed cultures of two organohalide respiring bacteria

12:00 to 13:00h Lunch break Mensa Augustusplatz

13:30 to 15:30 City Flash Point Tour PhD Students

15:30 – 17:00h Poster session II

17:00 – 19:00h Omics

Sebastien Cecillon (Université de Lyon ) - Bacteria Social Networks in Chlorinated Environments

Shanquan Wang (Sun Yat-Sen University, Guangzhou) - Genomics-directed isolation of PCB-dechlorinating Dehalococcoides

Olivia Molenda (University of Toronto) -  Eight new Dehalococcoides mccartyi genomes and a snap shot of both beneficial and parasitic DNA exchange in the KB-1 consortium

Dominique Türkowsky (UFZ Leipzig) - Functional proteomics approaches for in-depth analysis of regulation of reductive dehalogenation in Sulfurospirillum

Bill Moe (Louisiana State University) - Insights from the expanding number of species and genome sequences from the genus Dehalogenimonas


Wednesday March 29 (University Leipzig, Seminar Building Augustusplatz, Seminar Room 2


9:00 – 13:30 Ecology/Ecophysiology/Applied

Martin Elsner (Helmholtz Zentrum München) - Bridging the Gap between Reaction Flask and Environment: Isotope Analysis to Study Dehalogenation Mechanisms in Complex Systems

Louis Hermon (Université de Strasbourg) - Dichloromethane degradation in groundwater multi-contaminated by organohalides: coupling biomolecular and compound-specific isotope analyses

Daniel Buchner (Uni Tübingen) - Growth conditions do not affect carbon isotope fractionation for TCE dehalogenation by Desulfitobacterium hafniense strain Y51

Steffi Franke (UFZ Leipzig) - 1,2-Dichloroethane dehalogenation in Dehalococcoides mccartyi using compound-specific stable isotope analysis

Break 15 minutes

Simona Rossetti (IRSA-CNR, Italy) - In situ reductive dechlorination of PCBs: hunting for dechlorinating bacteria in contaminated marine sediments

Ernest Marco-Urrea (Universitat Autonoma de Barcelona) - Enhanced reductive dechlorination of chloroethenes in a contaminated aquifer in Barcelona (Spain)

Agnese Lai (Sapienza University of Rome) - Long-term Reductive Dechlorination of TCE in Bioelectrochemical reactor

Break 15 minutes

Ivonne Nijenhuis (UFZ Leipzig) - Ecophysiology of dehalogenation in the contaminated Bitterfeld-Wolfen megasite

Stefan Kruse (Uni Jena) - Complete dechlorination of tetrachloroethene to ethene by a co-culture of Sulfurospirillum multivorans and Dehalococcoides mccartyi

Nannan Jiang (University of Tennessee) - Engineered corrinoid biosynthesis pathway in Geobacter sulfurreducens sustains reductive dechlorination in Dehalococcoides


Closing Remarks, Lorenz Adrian/Gabriele Diekert