Friedrich-Schiller-Universität Jena

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Subproject 2

Biochemistry of respiration in Dehalococcoides strain CBDB1

Lorenz Adrian (UFZ Leipzig)


In subproject 2 we will investigate the biochemistry of organohalide respiration in Dehalococcoides strain CBDB1. Focus of our studies will be the biochemical description of reductive dehalogenases. These enzymes are membrane attached and responsible for electron transfer to halogenated compounds. Preliminary data shows the presence of a corrinoid cofactor and iron sulfur clusters. In strain CBDB1, reductive dehalogenase homologous genes make up the largest paralog group in the genome comprising a total of 32 different genes. A second goal of this project is the  identification of biochemical activities in some of these reductive dehalogenases.

Another topic of the project is the description of the electron flow to the dehalogenases via proposed membrane-bound electron mediators. Here, we will closely cooperate with subproject 3 which focuses on the description of hydrogenases and formate dehydrogenase in strain CBDB1.

sp2_dehalococc
Electron micrograph of Dehalococcoides mccartyi cells

Selected References:

Kublik A, Deobald D, Hartwig S, Schiffmann CL, Andrades A, von Bergen M, Sawers RG, Adrian L.
Identification of a multiprotein reductive dehalogenase complex in Dehalococcoides mccartyi strain CBDB1 suggests a protein-dependent respiratory electron transport chain obviating quinone involvement
Environ Microbiol. 2015 Dec 31., doi:10.1111/1462-2920.13200

Pöritz M., Goris T., Wubet T., Tarkka M.T., Buscot F., Nijenhuis I., Lechner U., Adrian L.
Genome sequences of two dehalogenation specialists - Dehalococcoides mccartyi strains BTF08 and DCMB5 enriched from the highly polluted Bitterfeld region
FEMS Microbiol Lett. 2013 Apr 19. (Epub ahead of print) doi:10.1111/1574-6968.12160

Tang S.,Chan W.W., Fletcher K.E., Seifert J., Liang X., Löffler F.E., Edwards E.A., Adrian L.
Functional Characterization of Reductive Dehalogenases Using Blue Native Polyacrylamide Gel Electrophoresis
Appl. Environ. Microbiol. 2012 Nov 30 (Epub ahead of print) doi: 10.1128/AEM.01873-12

 

Wagner A., Cooper M., Ferdi S., Seifert J., Adrian L.
Growth of Dehalococcoides mccartyi strain CBDB1 by reductive dehalogenation of brominated benzenes to benzene
Environ. Sci. Technol. 2012 Jul 16 (Epub ahead of print) doi: 10.1021/es3003519







Lorenz Adrian group